Inhibitory action of C22-fatty acids on DNA polymerases and DNA topoisomerases

Inhibitory action of C22-fatty acids on DNA polymerases and DNA topoisomerases.

We reported previously that unsaturated linear-chain fatty acids of the cis-configuration with a C18-hydrocarbon chain such as linoleic acid (cis-9, 12-octadecadienoic acid, C18:2) could potently inhibit the activity of mammalian DNA polymerases (Biochim Biophys Acta 1308: 256-262, 1996). In this study, we investigated the inhibitory effects of cis-type C22-fatty acids including cis-7,10,13,16,...

Antifungal activity of eupolauridine and its action on DNA topoisomerases.

The azafluoranthene alkaloid eupolauridine has previously been shown to have in vitro antifungal activity and selective inhibition of fungal topoisomerase I. The present study was undertaken to examine further its selectivity and mode of action. Eupolauridine completely inhibits the DNA relaxation activity of purified fungal topoisomerase I at 50 microg/ml, but it does not stabilize the cleavag...

DNA Topoisomerases

The double-helical nature of DNA and the anchoring of DNA to nuclear structures result in a number of topological problems during replication and transcription, mainly due to DNA-tracking polymerases and helicases. These activities cause the accumulation of positive supercoils ahead of the moving polymerase and negative supercoils behind it (Liu and Wang 1987; Brill and Sternglanz 1988; Giaever...

Inhibitory action of polyunsaturated fatty acids on Cdt1-geminin interaction.

A human replication initiation protein, Cdt1, is a central player in the cell cycle regulation of DNA replication, and geminin down-regulates Cdt1 function by direct binding. It has been demonstrated that Cdt1 hyperfunction resulting from Cdt1-geminin imbalance, for example, by geminin silencing with small interfering RNA, induces DNA re-replication and eventual cell death in some cancer-derive...

Inhibition of DNA polymerases in oncornaviruses by modified nucleic acids.